il 1ra anakinra  (MedChemExpress)

Journal: Cell reports

Article Title: Antibodies targeting the shared cytokine receptor IL-1 receptor accessory protein invoke distinct mechanisms to block all cytokine signaling

doi: 10.1016/j.celrep.2024.114099

Figure Lengend Snippet: (A) Cellular landscape of peritoneal lavage with no MSU exposure and after MSU exposure 6 h post in IL-1RAcP WT ( n = 11) and knockout (KO) ( n = 6) mice. The graph shows mean values. (B–D) Experimental design (B) for the MSUexperiment with administration of control (PBS), IL-1Ra, mIgG2a isotype control, or anti-IL-1RAcP antibody (mCAN10). (C) Leukocyte and neutrophil concentrations by flow cytometry in peritoneal lavage fluid after 6 h after MSU administration. The line is at the mean. Each symbol represents one mouse. (D) Cytokine profiles by Luminex from peritoneal lavage of IL-1Ra and anti-IL-1RAcP antibody mCAN10 treatments, normalized to vehicle and isotype controls, respectively. The graph shows mean and SEM. Statistical analysis was done using Mann-Whitney * p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001. In (A), the statistics above the bars show differences for total cells per milliliter, while the statistics inside the bars show differences for neutrophils and monocytes specifically. In (D), statistics are shown for mCAN10 vs. IL1Ra normalized to their vehicle and isotype controls (iso), respectively. Statistics not shown: decrease in concentrations of analytes for IL1Ra and mCAN10 vs. their respective controls; G-CSF (IL1Ra vs. PBS ***, mCAN10 vs. iso ***), IL-6 (IL1Ra vs. PBS **, mCAN10 vs. iso **), IL-5 (IL1Ra vs. PBS ns; mCAN10 vs. iso *), eotaxin (IL1Ra vs. PBS ns, mCAN10 vs. iso **), MIP1b (IL1Ra vs. PBS ns, mCAN10 vs. iso *), MCP1 (IL1Ra vs. PBS ns, mCAN10 vs. iso *), KC (Keratinocyte Chemoattractant/CXCL1) (IL1Ra vs. PBS ns, mCAN10 vs. iso ns).

Article Snippet: Kineret (Anakinra/IL-1Ra) , Sobi, Sweden , NA.

Techniques: Knock-Out, Control, Flow Cytometry, Luminex, MANN-WHITNEY

Journal: Cell reports

Article Title: Antibodies targeting the shared cytokine receptor IL-1 receptor accessory protein invoke distinct mechanisms to block all cytokine signaling

doi: 10.1016/j.celrep.2024.114099

Figure Lengend Snippet: (A) A cartoon of typical IL-1-family agonist signaling, demonstrating the cytokine IL-1 binding its primary receptor IL-1RI and recruiting the shared secondary receptor IL-1RAcP to form a signaling-competent ternary complex (i.e., cytokine/primary receptor/secondary receptor). (B) A cartoon of typical IL-1-family signaling antagonism, demonstrating the natural antagonist IL-1Ra binding to the primary receptor IL-1RI and precluding the recruitment of IL-1RAcP to form a signaling-competent ternary complex. In addition, a monoclonal antibody (mAb) is shown binding to IL-1RAcP and precluding the formation of a signaling-competent ternary complex. (C) Cell signaling assays of IL-1 α with the natural antagonist IL-1Ra, the antibody CAN10, and the antibody 3G5 with error bars denoting SEM. (D) Cell signaling assays of IL-1 β with the natural antagonist IL-1Ra, the antibody CAN10, and the antibody 3G5 with error bars denoting SEM. (E) Cell signaling assays of IL-33 with the natural antagonist sST2, the antibody CAN10, and the antibody 3G5 with error bars denoting SEM. (F) Cell signaling assays of IL-36 α with the natural antagonist IL-36Ra, the antibody CAN10, and the antibody 3G5 with error bars denoting SEM. (G) Cell signaling assays of IL-36 β with the natural antagonist IL-36Ra, the antibody CAN10, and the antibody 3G5 with error bars denoting SEM. (H) Cell signaling assays of IL-36 γ with the natural antagonist IL-36Ra, the antibody CAN10, and the antibody 3G5 with error bars denoting SEM.

Article Snippet: Kineret (Anakinra/IL-1Ra) , Sobi, Sweden , NA.

Techniques: Binding Assay

Journal: Cell reports

Article Title: Antibodies targeting the shared cytokine receptor IL-1 receptor accessory protein invoke distinct mechanisms to block all cytokine signaling

doi: 10.1016/j.celrep.2024.114099

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Kineret (Anakinra/IL-1Ra) , Sobi, Sweden , NA.

Techniques: Virus, Bacteria, Recombinant, Multiplex Assay, Luminex, Transfection, Construct, Software

Journal: Nature Communications

Article Title: IL-1β promotes adipogenesis by directly targeting adipocyte precursors

doi: 10.1038/s41467-024-51938-x

Figure Lengend Snippet: a – d Body weight development ( a ), body weight ( b ), fat pad mass ( c , d ) of 17-week-old chow and HFD-fed male mice. n = 12 WT-CD, n = 12 IL1R1-KO-CD, n = 14 WT-HFD, n = 14 IL1R1-KO-HFD. e – h Adipocyte size distribution ( e – h ) of 17-week-old chow and HFD-fed male mice. n = 10 IL1R1-WT-CD, n = 12 IL1R1-KO-CD ( e ). n = 14 per genotype ( f ). n = 12 IL1R1-WT-CD, n = 10 IL1R1-KO-CD ( g ). n = 14 IL1R1-WT-HFD, n = 13 IL1R1-KO-HFD (h). i – n Body weight development ( i ), body weight ( j ), fat pad mass ( k, l ) and adipocyte size distribution ( m, n ) of 17-week-old HFD-fed male mice treated with IL-1Ra (10 mg/kg bw daily for 14 days, n = 16 for both conditions). o , p Relative Il1r1 mRNA expression in adipocytes, CD45 – , and CD45 + stromal vascular cells isolated from gWAT ( o ) and scWAT ( p ) of 12-week-old chow-fed male WT mice (n = 5). q – s Experimental scheme of EdU tracing experiments (100 μg EdU/day, every 2 days, total of 4 injections) ( q ) and percentage of EdU + adipocyte nuclei isolated from gWAT ( r ) and scWAT ( s ) of IL1R1-KO mice and WT controls. gWAT IL1R1-KO and gWAT WT: chow diet (n = 12 per genotype); HFD (n = 14 per genotype). scWAT IL1R1-KO and scWAT WT: chow diet (n = 11 per genotype); HFD (n = 12 per genotype). t – v Experimental scheme of EdU tracing experiments (100 μg EdU/day, every 2 days, total 4 injections) and IL-1Ra therapy (10 mg/kg bw daily for 14 days) ( t ) and percentage of EdU + adipocyte nuclei isolated from gWAT ( u ) and scWAT ( v ) of HFD-fed WT mice. gWAT: saline and IL-1Ra (n = 16). scWAT: saline (n = 16); IL-1Ra (n = 13). Scale bar = 200 µm. Experimental schemes created with biorender.com. n = biological replicates. Data are shown as individual measurements and mean ± SEM. Statistical analyses were performed by: unpaired nonparametric two-tailed Mann-Whitney U test ( j – l , u , v ); one-way ANOVA and Šidák’s multiple comparison test ( o , p ); or two-way ANOVA and Šidák’s ( a , e – i , m , n ) or Fisher’s LSD ( b – d , r , s ) multiple comparison test. Source data are provided as a Source Data File.

Article Snippet: WT mice were: injected with 100 µg EdU every 2 days, for 7 days (day 1 to day 7); daily treated with 10 mg/kg body weight IL-1Ra (Anakinra, Kineret, Sobi, Germany) at 4–6 pm, for 14 days (day 1 to day 14); HFD-fed for 9 weeks (starting at day 3).

Techniques: Expressing, Isolation, Saline, Two Tailed Test, MANN-WHITNEY, Comparison

Journal: Nature Communications

Article Title: IL-1β promotes adipogenesis by directly targeting adipocyte precursors

doi: 10.1038/s41467-024-51938-x

Figure Lengend Snippet: a , b Representative images ( a ) and quantification ( b ) of lipid droplet accumulation in freshly FACS-sorted scWAT-derived human primary adipocyte progenitors differentiated for 13 days with IL-1β (5 ng/ml) (n = 3 donors). Scale bar = 100 μm. c , d Representative images ( c ) and quantification ( d ) of lipid droplet accumulation in hASCs differentiated for 9 days with indicated concentrations of IL-1β (10 ng/ml in c ) (n = 3 independent experiments with ≥ 4 replicates (34 datapoints for vehicle and 12 for the rest)). Scale bar = 100 μm. e Adipogenic gene expression on day 2 ( PPARG ) or 5 ( ADIPOQ and PLIN1 ) of differentiation in hASCs treated with IL-1β from start of differentiation (n = 4 independent experiments with 2 replicates). f Lipid droplet accumulation in hASCs differentiated for 9 days with IL-1β (10 ng/ml) and IL-1Ra (500 ng/ml) (n = 4 independent experiments with ≥ 4 replicates (33 datapoints for –IL-1Ra +vehicle and 16 for the rest)). g Adipogenic gene expression in hASCs 2 days after adipogenic induction +/– IL-1β (10 ng/ml) and IL-1Ra (500 ng/ml) (n = 4 independent experiments with 2 replicates). h , i Single-cell resolution image analysis of lipid droplet area in individual cells (hASCs differentiated for 9 days with 10 ng/ml IL-1β). Distribution of total area covered by lipid droplets within individual cells ( h ) and % of undifferentiated cells ( i ) (n = 4 analyzed wells per condition, each well containing around 10 4 cells). j Lipid droplet accumulation in hASCs differentiated for 9 days with IFN-γ (10 ng/ml), IL-6 (10 ng/ml), LPS (100 ng/ml), MCP-1 (20 ng/ml), TNF-α (10 ng/ml), IL-1β (10 ng/ml), or vehicle (n = 3 independent experiments with ≥ 4 replicates (14 datapoints for vehicle and 12 for the rest)). Statistical analyses by paired ( b ) or unpaired ( i ) two-sided t test, one-way ANOVA and Dunnett’s multiple comparisons test compared to vehicle ( d, e, j ) or IL-1β ( j ), two-way ANOVA and Šidák’s multiple comparisons test ( f , g ), or Mann-Whitney test ( h ). Data are represented as individual measurements and mean ± SEM, box-and-whisker plots (line inside box = median; box limits = first and third quartiles; whisker ends = minima and maxima), or violin plots. Source data are provided as a Source Data File.

Article Snippet: WT mice were: injected with 100 µg EdU every 2 days, for 7 days (day 1 to day 7); daily treated with 10 mg/kg body weight IL-1Ra (Anakinra, Kineret, Sobi, Germany) at 4–6 pm, for 14 days (day 1 to day 14); HFD-fed for 9 weeks (starting at day 3).

Techniques: Derivative Assay, Expressing, MANN-WHITNEY, Whisker Assay